Comparative Analysis of Structural and Dynamical Features of Ribosome Upon Association With mRNA Reveals Potential Role of Ribosomal Proteins

Ribosomes play a critical role in maintaining cellular proteostasis. The binding of messenger RNA (mRNA) to the ribosome regulates kinetics protein synthesis. To generate an understanding structural, mechanistic, and dynamical features mRNA recognition ribosome, we have analysed mRNA-protein interactions through structural comparison ribosomal complex presence absence mRNA. do so, compared 3-Dimensional (3D) structures components two assembly their differences because binding, using elastic network models network-based analysis. We observe that head region 30S subunit undergoes displacement rearrangement accommodate incoming find these changes are observed proteins lie far from interface, implying allostery. Further, perturbation response scanning, show S13, S19, S20 act as universal sensors sensitive inter network, upon with other initiation factors. Our study highlights significance identifies putative allosteric sites corresponding alterations structure and/or dynamics, regions away complex. Overall, our work provides fresh insights into association highlighting dynamics binding.